THE IDENTIFICATION OF SUGARCANE GENES BY RANDOM SEQUENCING OF cDNA CLONES
نویسنده
چکیده
Very little is known about sugarcane gene sequences. In this study, the aim is to identify sugarcane genes expressed in the leaf roll and the mature stem. Random DNA sequencing of cDNA clones allows the identification of genes and the development of Expressed Sequence Tags (ESTs). A total of 250 clones randomly selected from a leaf roll cDNA library and 112 from a mature stem library have been sequenced. Homology searches with sequences located in international databases have indicated a broad diversity of genes encoding proteins such as metabolic enzymes, structural proteins and regulatory factors. Preliminary analyses have indicated that although genes common to both the leaf roll and mature stem have been detected, several stress-related genes have been identified in the mature stem. Further studies will determine whether ESTs may be used to identify differentially expressed genes in sugarcane tissues.
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PATTERNS OF GENE EXPRESSION IN SUGARCANE MONITORED USING cDNA MACROARRAYS
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